The detection of Aflatoxin M1 and antibiotics in milk and dairy products is essential for ensuring food safety and quality. At the same time, milk adulteration needs to be monitored on order to prevent allergies and in some cases economical loss.

Mycotoxins are toxic substances produced by certain molds that can contaminate animal feed, including dairy feed. Aflatoxin B1 is a mycotoxin that is commonly found in corn. When contaminated corn is consumed by animals, the Aflatoxin B1 can be metabolized in their liver, producing a metabolite called Aflatoxin M1, which can then be excreted in the animal’s milk.

Antibiotics are commonly used in the dairy industry to treat or prevent infections in dairy animals. If antibiotics are present in milk at higher-than-permitted levels, they can pose a risk to human health.

Milk adulteration refers to the intentional or unintentional addition of substances or contaminants to milk that changes its composition or quality.

ProGnosis Biotech offers Bio-Shied ELISA and the novel Symmetric and Rapid Technology kits for Aflatoxin M1, antibiotics detection and adulteration in milk and dairy products. Our kits are validated by landers Research Institute for Agriculture, Fisheries and Food (ILVO).

By continuing to evolve its dairy testing portfolio, ProGnosis Biotech is demonstrating a commitment to excellence in food safety.

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Antibiotics are widely used for disease prevention in dairy farms. β-lactams and tetracyclines are the most often used antibiotics in dairy cattle management.

A number of analytical methods have been developed to monitor antibiotic residues in milk, including enzyme-linked immunosorbent assays (ELISAs) and lateral flow assays.

In order to monitor presence of antibiotics in milk, ProGnosis Biotech developed an immunochromatography test kit Symmetric B+T Series that helps farmers, truckers, and dairy laboratories rapidly detect whether samples are meeting maximum residue limits (MRLs) according to their regional requirements.

Symmetric B+T lateral flow test is designed to simultaneously detect and quantify the presence or absence of β-lactams and tetracyclines in cow, sheep, and goat milk in 5 minutes.

Chloramphenicol is a broad-spectrum antibiotic used to treat severe bacterial infections in humans, but it is not approved for use in food-producing animals. The presence of chloramphenicol in milk can pose a risk to human health, as it can cause aplastic anaemia, a potentially fatal condition.

As a solution, ProGnosis Biotech offers Bio-Shield Chloramphenicol ELISA kit, highly sensitive and specific that can accurately quantify the presence of chloramphenicol in milk samples in only 30 minutes.



Aflatoxins are toxic metabolites of significant concern to the dairy industry, generally produced by Aspergillus flavus, A. parasiticus and A. nomius. They can have immunosuppressive, mutagenic, teratogenic, and carcinogenic effects.

Aflatoxin M1 is a potent carcinogen that is toxic to humans and animals and is particularly dangerous to infants and young children. As a result, there is an established EU limit of 0,05 μg/kg (50 ppt) and 0.025 μg/kg (25 ppt) for infant food.

The most common methods for detecting Aflatoxin M1 are enzyme-linked immunosorbent assays (ELISAs) and lateral flow assays.

In order to satisfy all the milk industry markets, ProGnosis Biotech offers four different types of Aflatoxin M1 ELISA products: Bio-Shield Aflatoxin M1 Extra Sensitive (ES), Bio-Shield Aflatoxin M1 Fast, Bio-Shield Aflatoxin M1 Baby Food (BF), and Bio-Shield Aflatoxin M1 Ultra Fast Milk&Yougurt.

We also developed two types of immunochromatographic assays, Symmetric M1 which can quantitively detect Aflatoxin M1 in only 10 minutes, and it is specifically designed for Aflatoxin M1 EU market’s limits and regulations. While our Symmetric M1 1000 lateral flow test is recommended to be used by the countries where the allowed Aflatoxin M1 limits are above 0,05 μg/kg (50 ppt).



Milk adulteration can have profound health implications for consumers and can also result in economic losses for producers and processors.

Higher-priced milk with limited seasonal availability is commonly and fraudulently adulterated with lower milk prices from different dairy animals like cows. Adulterated milk, used in direct human consumption or in cheese manufacture (like feta, which is recognized as a Protected Designation of Origin, PDO), results in a final product inferior to that expected by the consumer. Due to unknown milk mixtures, significant changes in the final sensory characteristics and quality may occur.

In addition, cow’s milk allergy (CMA) is one of the most common food allergies, while Goat’s milk is also associated with a rare allergy disorder. Therefore, food labels must declare cow or goat milk as ingredients.

The presence of milk adulteration can be detected by toilsome methods such as chromatography and electrophoresis, but these methods require highly sophisticated and expensive equipment and are, therefore, of limited value for regular milk screening.

ProGnosis Biotech offers accurate and reliable methods to milk adulteration issues. Our ELISA Bio-Shield Cow, Bio-Shield Cow Cheese, Bio-Shield Goat, and Bio-Shield Goat Colostrum ELISA kits as well as the lateral flow products: Rapid Cow and Rapid Goat are an easy solution to dairy farms and industries to identify milk adulteration.